Results
| PMID | 17483545 |
| Gene Name | NFKB1 |
| Condition | Endometriosis (Peritoneal) |
| Association |
Associated |
| Population size | 36 |
| Population details | 36 biopsies of peritoneal endometriotic lesions |
| Sex | Female |
| Associated genes | NF-kappaB |
| Other associated phenotypes |
Peritoneal endometriosis |
|
Mol Hum Reprod. 2007 Jul;13(7):503-9. Epub 2007 May 5. Gonzalez-Ramos, Reinaldo| Donnez, Jacques| Defrere, Sylvie| Leclercq, Isabelle| Squifflet, Jean| Lousse, Jean-Christophe| Van Langendonckt, Anne Department of Gynecology, Universite Catholique de Louvain, Cliniques Universitaires St Luc, Avenue Hippocrate 10, 1200 Brussels, Belgium. Red (active), black and white endometriotic lesions are characteristic of peritoneal endometriosis. The transcription factor nuclear factor-kappa B (NF-kappaB) activates proinflammatory, proliferative and antiapoptotic genes in many cell types. To determine whether NF-kappaB is activated in peritoneal endometriosis in women, and further ascertain the differential inflammatory status of endometriotic implants, NF-kappaB activation and intercellular adhesion molecule (ICAM)-1 expression were investigated in peritoneal endometriotic lesions according to their type. Furthermore, p65 and p50 subunits of active NF-kappaB dimers were evaluated in endometriotic lesions to gain some insight into NF-kappaB-implicated pathways. Thirty-six biopsies of peritoneal endometriotic lesions were analyzed. Constitutive NF-kappaB activation, involving p65- and p50-containing dimers, was demonstrated in peritoneal endometriotic lesions by electrophoretic mobility shift assays and supershift analyses, as well as NF-kappaB (p65) DNA-binding activity immunodetection assays. NF-kappaB activation and ICAM-1 expression (evaluated by immunoblotting) were significantly higher in red lesions than black lesions, whereas IkappaBalpha (NF-kappaB inhibitory protein) expression was constant, as shown by western blot analysis. This is the first study to demonstrate constitutive NF-kappaB activation in peritoneal endometriosis in women. NF-kappaB activation and ICAM-1 expression in red lesions confirm the more extensive inflammatory pattern of these lesions compared with black lesions. The involvement of p50/p65 dimers in NF-kappaB activation suggests implication of the classic NF-kappaB activation pathway, making it an attractive therapeutic target in endometriosis. Mesh Terms: Adolescent| Adult| Endometriosis/*metabolism/pathology| Female| Humans| I-kappa B Proteins/metabolism| Intercellular Adhesion Molecule-1/metabolism| NF-KappaB Inhibitor alpha| NF-kappa B/*metabolism| Peritoneal Diseases/*metabolism/pathology| Pe |
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